Cell having ability of differentiating automatically pulsating cardiomyocyte isolated from tongue tissue and method for cell culture and differentiation induction

舌組織から単離された、自動拍動する心筋細胞に分化する能力を有する細胞および細胞の培養、分化誘導法

Abstract

【課題】自己舌組織から、拍動する心筋細胞を創生するための細胞、細胞の培養、分化誘導法の提供。再生医学に多大な貢献をする。 【解決手段】バイオプシーによってヒトからも採取可能な組織、特に自己舌組織幹細胞から、SCA1陽性でCD34の細胞を採取し、脱メチル化剤を用いずに、血小板由来増殖因子や上皮細胞増殖因子のような成長因子を含む培地の使用と、培養における細胞外環境因子として遠心負荷をかける方法によって、拍動する心筋細胞を誘導する。 【選択図】図8
<P>PROBLEM TO BE SOLVED: To obtain cells for creating a pulsating cardiomyocyte from self-tongue tissue, to provide a method for cell culture and differentiation induction and to greatly contribute to regeneration medicine. <P>SOLUTION: A pulsating cardiomyocyte is induced by collecting CD34 cells of SCA1 positive from tissue collectable even from human by a biopsy, especially from self-tongue stem cells, using a medium such as a growth factor such as a blood platelet-derived growth factor and an epithelial cell growth factor without using a demethylation agent and by a method for applying centrifugal loading as an extracellular environmental factor in culture to the cells. <P>COPYRIGHT: (C)2005,JPO&NCIPI

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    CN-102229911-ANovember 02, 2011山西医科大学Sca-1<sup>+</sup>/CD34<sup>-</sup>子宫干细胞及其分离方法
    CN-102229911-BSeptember 18, 2013山西医科大学Sca-1+/CD34- uterine stem cells and separation method thereof
    EP-2457994-A1May 30, 2012Daiichi Sankyo Company, LimitedProcédé pour l'induction de différentiation de cellules souches pluripotentes dans des cardiomyocytes
    JP-WO2014104364-A1January 19, 2017国立大学法人京都大学人工多能性幹細胞、心筋細胞又はその前駆細胞の製造方法
    US-8293529-B2October 23, 2012Daiichi Sankyo Company, LimitedMethod for inducing differentiation of pluripotent stem cells into cardiomyocytes
    WO-2007126077-A1November 08, 2007Asubio Pharma Co., Ltd.Procédé pour induction de la différenciation de cellules du myocarde à partir de cellules souches pluripotentes
    WO-2014104364-A1July 03, 2014国立大学法人京都大学Méthode de production de cellules souches pluripotentes induites, cardiomyocytes ou cellules précurseurs correspondantes